Journal: Respiratory research

Article Title: ITIH4 attenuates acute lung injury by Fe-containing particulate matter in mice via Hippo pathway in type II alveolar epithelial cells.

doi: 10.1186/s12931-025-03256-z

Figure Lengend Snippet: Fig. 2 A Schematic diagram of 7 weeks old B6.Sftpc-CreERT2;Ai14(RCL-tdT)-D mice pre-treated with Tamoxifen injection were intratracheally administered with either phosphate buffered saline (PBS) or diesel exhaust particle (DEP). After 1 h, the DEP-exposed mice were intranasally administered with recombinant ITIH4 (rITIH4) protein. B t-stochastic neighbor embedding (t-SNE) plot of all cells colored by their cellular identity and percentages of each cells cluster in lung of B6.Sftpc-CreERT2;Ai14(RCL-tdT)-D mice in control, DEP, and DEP with rITIH4 treatment groups. C t-SNE plot, percentages of cells, and (D) heatmap displaying the gene expressions of type II alveolar epithelial cell (AECII) by Epcam-positive (Epcam+) and Sftpc-positive (Sftpc+) cells within the B cell, brush cell, and epithelial cell cluster (pink), as well as the Axin2-Palpha + cell, dividing T cell, endothelial cell, epithelial cell, myofibroblast, smooth muscle cell, and T cell cluster (green). E t-SNE plot of AECII cells (Epcam+ Sftpc+) within the B cell, brush cell, and epithelial cell cluster and (F) dot plot of gene expressions related to Hippo pathway in control, DEP, and DEP with rITIH4 treatment groups. The depth of the color indicates log2 fold changes (red: positive fold change; blue: negative fold change). The size of the point indicates the percentage of cells in the group. G Gene ontology enrichment analysis related to biological processes (BP), cellular component (CC), and molecular function (MF). The depth of the color indicates the activation odds ratio. The size of the point indicates the p-values of the enrichment analysis

Article Snippet: One hour after exposure, the mice were intranasally administered with 50 μL of either sterilized PBS or 12.5 μg/kg recombinant human ITIH4 (rITIH4) (CSB-EP617923HU, Cusabio, Houston, TX, USA) [21, 22].

Techniques: Injection, Saline, Recombinant, Control, Activation Assay

Journal: Respiratory research

Article Title: ITIH4 attenuates acute lung injury by Fe-containing particulate matter in mice via Hippo pathway in type II alveolar epithelial cells.

doi: 10.1186/s12931-025-03256-z

Figure Lengend Snippet: Fig. 4 Violin plot of gene expressions (presented in log2 fold changes) in type II alveolar epithelial cells (AECII) Epcam+ Sftpc+ cells by single-cell RNA in lungs of B6.Sftpc-CreERT2;Ai14(RCL-tdT)-D mice in control, diesel exhaust particle (DEP), and DEP with rITIH4, representative western blot images, quantitative analysis of the western blot images with normalization to β-actin compared to control group, and representative immunofluorescent staining of lungs of mice in control, control with rITIH4, DEP, DEP with rITIH4, soluble iron (FeCl3), and FeCl3 with rITIH4 groups. A Violin plot of Cdh1 and Ctnna1, western blot images of E-cadherin and ⍺-catenin, and immunofluorescent staining with SPC (red), E-cadherin (purple), and ⍺-catenin (green) (nuclear staining with DAPI in blue). B Violin plot of Yap1, western blot images of YAP and p-YAP, and immunofluorescent staining with SPC (red), YAP (green), and p-YAP (purple) (nuclear staining with DAPI in blue). C Violin plot of Wwtr1, western blot images of TAZ and p-TAZ, and immunofluorescent staining with SPC (red), TAZ (purple), and p-TAZ (green) (nuclear staining with DAPI in blue) (scale bar = 50 μm). *p < 0.05

Article Snippet: One hour after exposure, the mice were intranasally administered with 50 μL of either sterilized PBS or 12.5 μg/kg recombinant human ITIH4 (rITIH4) (CSB-EP617923HU, Cusabio, Houston, TX, USA) [21, 22].

Techniques: Control, Western Blot, Staining

Journal: Respiratory research

Article Title: ITIH4 attenuates acute lung injury by Fe-containing particulate matter in mice via Hippo pathway in type II alveolar epithelial cells.

doi: 10.1186/s12931-025-03256-z

Figure Lengend Snippet: Fig. 5 A Violin plots (presented in log2 fold changes) of Trf, Trfc, and Map1 lc3b in type II alveolar epithelial cells (AECII) Epcam+ Sftpc+ cells by single-cell RNA of B6.Sftpc-CreERT2;Ai14(RCL-tdT)-D mice lungs in control, diesel exhaust particle (DEP), and DEP with recombinant ITIH4 (rITIH4). B representative western blot images and quantitative analysis of the western blot images with normalization to β-actin compared to control group, and representative immunofluorescent staining of lungs of mice in control, control with rITIH4, DEP, DEP with rITIH4, soluble iron (FeCl3), and FeCl3 with rITIH4 groups. *p < 0.05

Article Snippet: One hour after exposure, the mice were intranasally administered with 50 μL of either sterilized PBS or 12.5 μg/kg recombinant human ITIH4 (rITIH4) (CSB-EP617923HU, Cusabio, Houston, TX, USA) [21, 22].

Techniques: Control, Recombinant, Western Blot, Staining